{"id":843,"date":"2020-12-28T10:21:37","date_gmt":"2020-12-28T09:21:37","guid":{"rendered":"http:\/\/www.posi-lab.fr\/?p=843"},"modified":"2025-02-05T22:58:21","modified_gmt":"2025-02-05T21:58:21","slug":"high-resolution-typing-of-staphylococcus-epidermidis","status":"publish","type":"post","link":"https:\/\/www.host-pathogen-biology.fr\/index.php\/2020\/12\/28\/high-resolution-typing-of-staphylococcus-epidermidis\/","title":{"rendered":"High resolution typing of Staphylococcus epidermidis"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">28\/12\/2020 .<br><strong>High resolution typing of\u00a0<em>Staphylococcus epidermidis<\/em>\u00a0based on cgMLST to investigate the hospital spread of multidrug resistant clones<\/strong>. <br><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33328176\/\">Jamet <em>et al <\/em>J Clin Microbiol 2020 Dec 16<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">News linked to <a href=\"http:\/\/www.posi-lab.fr\/index.php\/2020\/04\/09\/anne-jamet\/\">Anne Jame<\/a><\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"500\" height=\"419\" src=\"http:\/\/www.posi-lab.fr\/wp-content\/uploads\/2020\/12\/JClinMicro_AJ2021.jpg\" alt=\"JClinMicro_AJ2021\" class=\"wp-image-844\" srcset=\"https:\/\/www.host-pathogen-biology.fr\/wp-content\/uploads\/2020\/12\/JClinMicro_AJ2021.jpg 500w, https:\/\/www.host-pathogen-biology.fr\/wp-content\/uploads\/2020\/12\/JClinMicro_AJ2021-300x251.jpg 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\" id=\"p-2\"><em>Staphylococcus epidermidis<\/em>&nbsp;is a pathogen emerging worldwide as a leading cause of health-care associated infections. A standardized high-resolution typing method to document transmission and dissemination of multidrug-resistant&nbsp;<em>S. epidermidis<\/em>&nbsp;strains is needed. Our aim was to provide a core genome multilocus sequence typing (cgMLST) typing scheme for&nbsp;<em>S. epidermidis<\/em>&nbsp;to improve the international surveillance of&nbsp;<em>S. epidermidis<\/em>.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\" id=\"p-3\">We defined a cgMLST scheme based on 699 core genes and used it to investigate the population structure of the species, and the genetic relatedness of isolates recovered from infants hospitalized in several wards of a French hospital. Our results show the long-lasting endemic persistence of&nbsp;<em>S. epidermidis<\/em>&nbsp;clones within and across wards of hospitals and demonstrate the ability of our cgMLST approach to identify and track these clones.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\" id=\"p-4\">We made the scheme publicly available through the Institut Pasteur BIGSdb server (<a href=\"http:\/\/bigsdb.pasteur.fr\/epidermidis\/\">http:\/\/bigsdb.pasteur.fr\/epidermidis\/<\/a>). This tool should enable international harmonization of the epidemiological surveillance of multidrug-resistant&nbsp;<em>S. epidermidis<\/em>&nbsp;clones. By comparing gene distribution among infection and commensal isolates, we also confirmed the association of the&nbsp;<em>mecA<\/em>&nbsp;locus with infection isolates and of the&nbsp;<em>fdh<\/em>&nbsp;gene with commensal isolates.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>28\/12\/2020 .High resolution typing of\u00a0Staphylococcus epidermidis\u00a0based on cgMLST to investigate the hospital spread of multidrug resistant clones. Jamet et al J Clin Microbiol 2020 Dec 16 News linked to Anne &#8230;<\/p>\n","protected":false},"author":1,"featured_media":844,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[31,27],"tags":[],"class_list":["post-843","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-award-grant-older-news","category-tsa"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>High resolution typing of Staphylococcus epidermidis - PoSI-Lab<\/title>\n<meta name=\"description\" content=\"High resolution typing of Staphylococcus epidermidis, PoSI-Lab, INEM, Institut Necker Enfants Malades, Anne Jamet, Inserm\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" 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